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Fig. 6. Tissue-specific and head-independent phosphorylation of p38 MAPK at
0°C. (A) Red-eye stage nondiapausing pharate adults were exposed at
0°C for 2 h, and specific tissues were dissected. Tissue proteins were
analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK
antibodies. (B) Red-eye stage nondiapausing pharate adults were ligated
between the head and thorax with a nylon thread and exposed at 0°C.
Protein samples from the posterior part were analyzed using western blotting
with anti-phospho-p38 and anti-total p38 MAPK antibodies. NT, no treatment;
0°C, held at 0°C for 2 h.
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