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Fig. 5. Phosphorylation of p38 MAPK in diapause-programmed flies reared at 20°C
and exposed to 0°C. (A) Feeding 3rd instar to day of pupariation, (B)
diapausing pupae 0 to 50 days after pupariation and (C) day 20 diapause pupae
to which 5 µl hexane was applied to elicit diapause termination. Larvae or
pupae were held at 0°C for 2 h, and protein samples were prepared. Whole
body proteins were analyzed using western blotting with anti-phospho-p38 and
anti-total p38 MAPK antibodies. FL, 3rd-instar feeding larvae;
W0–3, day 0–3 wandering 3rd-instar larvae; P0–50,
day 0–50 after pupariation; NT, no treatment; 0°C, held at 0°C
for 2 h. Red-eye stage nondiapausing pharate adults reared at 25°C were
used as controls.
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