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Fig. 4. Development-specific phosphorylation of p38 MAPK in nondiapausing flies at
0°C. Flesh flies were reared at a nondiapausing condition of (A) 25°C
or (B) 20°C. Larvae or pupae were held at 0°C for 2 h, and protein
samples were prepared. Fly proteins were analyzed using western blotting with
anti-phospho-p38 and anti-total p38 MAPK antibodies. FL, 3rd-instar
feeding larvae; W0-2, day 0–2 wandering 3rd-instar larvae;
P0–12, day 0–12 after pupariation; NT, no treatment; 0°C, held
at 0°C for 2 h. Red-eye stage nondiapausing pharate adults reared at
25°C were used as controls.
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