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Fig. 3. Temporal profiles of activation and decay of p38 MAPK phosphorylation in
red-eye stage nondiapausing pharate adults. (A) Exposure to 0°C for
various durations. (B) Exposure to 0°C for 10 min and then transferred to
25°C. (C) Exposure to 0°C for 2 h and then transferred to 25 and
–10°C for 2 h, or held for an additional 2 h at 0°C. (D)
Exposure to 0°C for 2 h and then transferred to 25°C for various
durations. Flies held at 25°C were used as controls. Fly proteins were
analyzed using western blotting with anti-phospho-p38 and anti-total p38 MAPK
antibodies.
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