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Figure 3


Fig. 3. Specific binding of nuclear proteins to ORE. The specificity of nuclear extracts to radiolabeled ORE1 binding under different experimental conditions is shown. Control (not challenged; column 1) and 8 h post in vivo hypo-osmotic stress (column 2); competitive displacement in the presence of a 100-fold excess of unlabeled ORE-1 oligonucleotide (column 3); formation of a nonspecific complex using a random oligonuclotide (column 4), and control without nuclear extract (column 5). The arrow shows the putative OREBP–ORE complex.





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