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Figure 3


Fig. 3. (A) Stage-dependent changes in fictive lung burst frequency during noradrenaline (NA; 5 µmol l–1) application. The histograms (left) show the mean data and the trigeminal neurograms ({int}V, trigeminal nerve; right) show representative recordings obtained under baseline conditions and following NA bath application. Data are reported for both stage groups (pre-metamorphic: grey bars, N=6; and adults: black bars, N=6). (B,C) The same experiment was performed in the presence of the GABAA/glycine antagonist mixture (bicuculline 1.25 µmol l–1/strychnine 1.5 µmol l–1) in both stage groups. For these experiments, representative trigeminal neurograms showing respiratory-related activity observed under each condition are shown below the histograms. Values are means ± s.e.m. *Values statistically different from baseline at P<0.05; {dagger}value statistically different from corresponding mixture value at P<0.05.





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