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Fig. 1. (A) Experimental protocol for low temperature and spring–summer
simulation experiment with mussel Mytilus edulis and oyster
Crassostrea virginica. Circles indicate dates of clearance rate
measurements and lipid sampling. Routine
O2 was measured
on 12 April, 4 May and 31 May and
O2min was
measured on 8 June after starving the animals used for routine
O2. (B)
Schematic of the split-split plot experimental design. T°C=9, 4
or 0. See Materials and methods for further details.