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Fig. 1. Experimental apparatus used to measure oxygen consumption and work. Muscle
(mu) was mounted in a cylindrical glass chamber (bound by broken lines)
surrounded by a temperature controlled water jacket (wj). One end of the
muscle was attached to a rigid, stainless-steel arm (ra) secured to the
stainless-steel chamber lid (cl). The other end was attached to a
stainless-steel pin (pn) that passes through a narrow aperture in the lid and
was connected to the arm of an ergometer (er). A glass-encapsulated magnetic
stir bar (sb) was used to mix the saline in the chamber. An oxygen probe
(op) enters the chamber through a sealed side port. A temperature
probe (tp) was placed adjacent to the chamber. To flush the chamber, saline
entered through a port in the bottom (arrow) and exited through the hole in
the chamber lid; diameter of holes in chamber lid and bottom are exaggerated
for illustration purposes. Chamber lid and bottom formed a tight seal with the
walls of the chamber using rubber O-rings. The rigid arm and pin connected to
the ends of the muscle were attached to fine magnet wires outside the chamber
(not shown) and used to stimulate the muscle. The rigid arm was insulated
along its length with polyethylene tubing to minimize stray current in the
chamber.
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