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Figure 4


Fig. 4. Differential expression of RH1A and RH1B opsins. (A) Agarose gel electrophoresis separation of PCR products using RH1A- or RH1B-specific primers and cDNA from 300 m and 950 m caught fish. Note presence of RH1A transcripts in fish caught at both depths but RH1B transcripts only in fish caught at 950 m. (B) Relative levels of expression of RH1A and RH1B in two fish caught at 300 m and one at 950 m. Agarose gel electrophoresis separation of PCR products amplified using forward and reverse primers to identical sequences in RH1A and RH1B sequences. Amplified fragments are arrowed. Note smaller digested SalI fragments identifying RH1B transcripts only in cDNA from 950 m fish.





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