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Fig. 3. Identification of RH2 opsin. (A) Amino acid sequence of S. analis
RH2 opsin aligned with the orthologous sequence from Metriaclima
zebra. Note the additional Ser residues near the C terminus of the S.
analis sequence. (B) Phylogenetic tree of RH2 opsin nucleotide sequences
generated by the neighbour-joining method. The RH1 sequence from Carassius
auratus was used as an outgroup. All sequences were aligned with
ClustalV. Accession numbers for the sequences (in order from the top of the
tree) are as follows: DQ088651, DQ088650, AY296739, Y18680, AY631037,
AY771352, NM_001033712, AF38524, EF517406, AY599603, DQ088652, AB223053,
AY214132, AF425076, AB098703, AB098704, AB087805, AB087806, AB087807,
AB087808, L11865, AB110603, L11866, AB110602, L11863.