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Files in this Data Supplement:
Movie 1. Split-screen showing external view of fish synchronized with endoscopic view of tissue pad on oropharyngeal roof (left of endoscopic image) and branchial arches (right of endoscopic image). The anterior of the fish is at the bottom right of the endoscopic image. As water is sucked into the mouth during suspension feeding, the food particles travel towards the esophagus at the upper left of the endoscopic image. This video sequence was recorded at 125 frames s−1 and is 600 ms in duration; every second or third frame is shown.
Movie 2. Endoscopic view of branchial arches on the left. As the sequence begins during a feeding pump, food particles are moving towards the esophagus at the top of the image. The gill rakers are obscured by a mucus aggregate that is attached to the rakers in the center of the field of view. At frame 3100, stage 1 of a reversal begins and the mucus aggregate lifts from the arches. By frame 3175, stage 2 has begun and the mucus aggregate exits from the field of view in a posterior direction. At frame 3209, the individual triangular rakers can be seen protruding from branchial arches 1 and 2. The small stationary white dots at the left of the image are broken endoscopic light fibers. This video sequence was recorded at 125 frames s−1 and is 1050 ms in duration; every third or fourth frame is shown.
Movie 3. Split-screen showing external view of fish synchronized with endoscopic view. The images in Fig. 1 were digitized from this video sequence. The anterior of the fish is at the bottom of the endoscopic view, and is at the right of the external view. At the beginning of the sequence, food is introduced in front of the oral jaws through a tube (Fig. 1A). At frame 3804, the oral jaws have abducted, and food particles can be seen passing through the endoscopic field of view as the particles travel towards the esophagus at the upper left (Fig. 1B). As stage 1 of a reversal occurs in frames 3815−3834, a mucus aggregate that has been resting on the arches at the left of the field of view subsequently lifts from the arches (Fig. 1C). As stage 2 of a reversal clears mucus from the field of view, the row of lateral gill rakers is visible on the third branchial arch in frame 3841 (large bright structures in center of field of view, Fig. 1D). The small stationary white dots at the left of the image are broken endoscopic light fibers. This video sequence was recorded at 125 frames s−1 and is 1100 ms in duration; every third or fourth frame is shown.
Movie 4. Endoscopic view of tissue pad on oropharyngeal roof (left) and branchial arches (right). The anterior of the fish is at the bottom of the endoscopic image. As water is sucked into the mouth during suspension feeding, the food particles travel parallel to the branchial arches as the particles are transported towards the esophagus at the top of the endoscopic image. This video sequence was recorded at 125 frames s−1 and is approximately 800 ms in duration; every second or third frame is shown.
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