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Fig. 5. Steady-state activation of the chloride-conductance,
GCl,H. (A) Mean GV relationship
at two different internal Cl concentrations. Paired
measurements of currents and of ECl were performed in six
fibres after moderate and strong Cl loading of the fibre
(the respective recordings were performed 1 and 5 min after impalement; for
details of the approach and for the voltage-clamp protocols employed, cf.
Materials and methods). Currents were converted to conductance, G.
The conductance is greatly enhanced at raised
[Cl]i. This effect is largely because of the
increase in maximal conductance. (B) Normalised GV
relationships for the data shown in A. The rise in [Cl]i led to
some positive shift of the curve on the voltage axis. Curves in A and B are
Boltzmann-fits. For further analysis, cf.
Table 2. Error bars indicate
s.e.m.