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Fig. 7. Effects of chronic exposure of D. melanogaster larvae to dietary
salicylate on salicylate influx across three segments of the isolated gut.
Fluxes were measured using the scanning ion electrode technique as described
in the Materials and methods. Gut segments were bathed in 30 mmol
l1 Cl saline containing 100 µmol
l1 salicylate. Flux for each segment of each larva was
calculated as the mean value for three sites separated by 480 µm for the
midgut and 200 µm for the ileum and rectum. Solid and hatched bars indicate
mean ± s.e.m. for 45 larvae in the control and experimental
group, respectively. Experimental larvae were raised for 10 days on a 10 mmol
l1 salicylate-enriched diet. Control larvae were raised on a
salicylate-free diet. No significant differences between groups were observed
(P>0.05).