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Fig. 6. Effects of 10 µmol l–1 leucokinin I on the rate of
fluid secretion by the main segment of isolated Malpighian tubules set up in
the Ramsay assay. The first secreted droplet was collected at 30 min (solid
bars) and the second secreted droplet (hatched bars) was collected 30 min
after adding leucokinin I. Experimental larvae were raised for 10 days on a 10
mmol l–1 salicylate-enriched diet. Control larvae were raised
on a salicylate-free diet. Values are means ± s.e.m.
(N=7–9 tubules). Asterisks indicate significant differences in
fluid secretion rate before and after adding leucokinin are indicated
(***P<0.001; paired t-test,
N=7–9).
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