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Fig. 3. Mechanosensory neurons affect the frequency but not the shape of the
forward-swimming gait. (A) The shape of the forward swimming gait of
mec-4(d) (light gray) and mec-6(u450) (dark gray) mutant
worms does not vary with mechanical load. The solid line shows the line fit to
wild-type data from Fig. 2A.
(B) The temporal frequency of the swimming gait of mec-4(d) and
mec-6(u450) mutants decreases with increasing mechanical load but is
offset to higher temporal frequencies than in wild-type worms. The solid line
shows the line fit to wild-type data from
Fig. 2B. Each measurement in A
and B corresponds to data from 10-15 worms and 30-60 s of video (means
± 1 s.e.m.). (C) The shape of the forward-swimming gait of young adult
worms in 1% methylcellulose is unaffected by the mec-4(d) or
mec-6(u450) mutations or by ablation of the ALM or PLM touch receptor
neurons. The number of worms analyzed in each measurement is shown in
parentheses. Standard deviation errors bars, typically ±5% of the mean
value of each measurement, are smaller than the data points. Neither
mec-4(d) or mec-6(u450) mutants are distinguishable from
wild-type (P>0.01). None of the laser-ablated worms are
distinguishable from the mock surgical controls, in which worms were prepared
for laser surgery but not irradiated (P>0.01). (D) The temporal
frequency of the swimming gait of young adult worms is significantly increased
by mutation or laser ablation. Error bars indicate one standard deviation.
Differences between mutant and wild-type and between laser-ablated worms and
mock surgical controls are indicated at P<0.01 and at
P<0.001.
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