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Fig. 2. Effects of fasting (45 days) on the btGLUT4 protein content of red muscle
and white muscle from brown trout. (A) Immunodetection of btGLUT4 and actin
protein in total membrane preparations from red and white muscle. 25 µg of
protein was loaded in each lane, subjected to SDS-PAGE and immunoblotted using
antibodies against okGLUT4 and chicken actin. (B) Densitometric analysis of
the btGLUT4/actin ratios from red muscle and white muscle. Values are means
± s.e.m. and are referred to the fed group (control), which was set to
1 (N=4-5). *Significant differences compared with the
control group for each type of muscle (P<0.01).
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