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Fig. 3. Changes in mean cellular haemoglobin concentration (MCHC) as a function of
time in washed red blood cells (RBCs) in the presence of 10–4
mol l-1 ouabain. The dotted line indicates osmotic shrinkage of the
RBCs following addition of sucrose (calculated to yield 25% shrinkage). (A)
Oxygenated control (
) and shrunken (
) as well as deoxygenated
control (
) and shrunken (•) RBCs. (B) Difference in MCHC between
shrunken and control cells under oxygenated () or deoxygenated (•)
conditions. The dashed and the dash-dot-dashed lines indicate 50% recovery
from initial change in MCHC upon shrinkage in oxygenated and deoxygenated
RBCs, respectively. (C) Deoxygenated control and shrunken RBCs with and
without transport inhibitors. Control cells (), shrunken cells (•),
shrunken cells treated with 10–4 mol l-1 amiloride
(
) or with 10–5
mol l-1 bumetanide
(
). * indicates mean
values that are statistically different from the respective time zero value;
indicates mean values that are statistically different
from shrinkage under deoxygenated conditions; 
indicates
values for deoxygenated control RBCs that are statistically different from
values for oxygenated control RBCs. N=5, P<0.05.
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