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Figure 3


Fig. 3. Western blots showing the effect of superoxide dismutase (SOD) and catalase (CAT) on CoCl2-induced phosphorylation of p38-MAPK (Ai), MAPKAPK2 (Aii) and Hsp27 (Aiii) after CoCl2 treatment. The phosphorylated forms of the kinases were detected in extracts (50 µg of protein) from control hearts (control) or hearts perfused for 15 min with 500 µmol l-1 of CoCl2 in the presence or absence of SOD (12.5 U ml-1) and CAT (150 U ml-1). The effect of SOD and CAT alone were also assessed. An anti-actin antibody was used as a control for equal protein loading (Aiv). Densitometric analysis of phospho-p38 (B), phospho-MAPKAPK2 (C) and phospho-Hsp27 (D) was performed by laser scanning. Western blots shown are representative of at least three independent experiments; data are means ± s.e.m. for at least three independent experiments. *P<0.001 vs control (untreated) hearts; {dagger}P<0.001 vs CoCl2-treated hearts.





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