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Figure 2


Fig. 2. Western blots showing the effect of Trolox (Tr) and Lipoic acid (LA) on CoCl2-induced phosphorylation of p38-MAPK (Ai), MAPKAPK2 (Aii) and Hsp27 (Aiii) after CoCl2 treatment. The phosphorylated forms of the kinases were detected in extracts (50 µg of protein) from control hearts (control) or hearts perfused for 15 min with 500 µmol l-1 of CoCl2 in the presence or absence of TR (10 µmol l-1) and LA (10 µmol l-1). The effect of Tr and LA alone was also assessed. An anti-actin antibody was used as a control for equal protein loading (Aiv). Densitometric analysis of phospho-p38 (B), phospho-MAPKAPK2 (C) and phospho-Hsp27 (D) was performed by laser scanning. Western blots shown are representative of at least three independent experiments; data are means ± s.e.m. for at least three independent experiments. *P<0.001 vs control (untreated) hearts; {dagger}P<0.05 vs CoCl2-treated hearts.





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