Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Percentage survival of (A) 4 d.p.f. and (B) 8 d.p.f. embryos of A. limnaeus exposed to anoxia and a range of sodium sulfite concentrations. No significant effect of sulfite concentration on survival of anoxia was detected. Symbols represent mean ± s.e.m. (N=3).

• Supplemental Figure S2 -

  Fig. S2. The lethal time (LT₅₀) for anoxia is not affected by increasing the amount sodium sulfite between 0.25 and 2.5 mg ml⁻¹ in 4 d.p.f. (filled bars) or 8 d.p.f. (open bars) embryos of A. limnaeus. Values are means ± s.e.m. (N=3). No statistical differences were found between the treatment groups within each developmental stage.

• Supplemental Figure S3 -

  Fig. S3. Nuclear magnetic resonance (NMR) analysis of metabolites from diapause II embryos extracted in TCA. (A) One-dimensional ¹H NMR and (B) two-dimensional ¹H TOCSY (total correlation spectroscopy) analyses confirm the compounds and the general ratios of metabolites reported for the GC−MS analysis. Ala, alanine; GAB, γ-aminobutyrate; Gln, glutamine; Glu, glutamate; Lys, lysine; Thr, threonine; Val, valine.

• Supplemental Figure S4 -

  Fig. S4. One-dimensional ¹H NMR spectra of prediapause, hydrated diapause II, and dehydrated diapause II embryo perchloric acid extracts. These spectra corroborate the GC−MS analysis presented in this paper, but also identify glycinebetaine and phosphorylcholine as metabolites that may be present and warrant future attention. Abbreviations are the same as in Fig. S3.