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Figure 9


Fig. 9. Southern blot analysis of degradation of pDNA in cultures cod atrial endocardial endothelial cells (aEEC). Monolayer cultures were incubated with 10 µg native pDNA and cells were solubilised after 1, 4, 24, 48 h. SC, super coiled topoform; OC, open-circular topoform; L, linear topoform. Open-circular and linear topoforms of pDNA (R70pRomiLuc) were detectable after 1 h. After 24 h, no intact pDNA was detected in the cells.





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