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Figure 5


Fig. 5. Mitochondrial oxygen uptake as a function of myoglobin concentration using NADH (1 mmol l–1) as the sole substrate. For this experiment mitochondria were prepared by a less stringent procedure, making the mitochondria able to use exogenous NADH. Polarographically determined specific oxygen uptakes of this mitochondrial preparation were 134 and 412 mol O2 (mol cytochrome aa3)–1 min–1 using glutamate/malate or NADH (1 mmol l–1), respectively, as substrate. The latter may be compared to the rate exhibited by mitochondria fully uncoupled by exposure to carbonyl cyanide m-chlorophenylhydrazone (CCCP), 700 mol O2 (mol cytochrome aa3)–1 min–1.





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