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Fig. 4. Mitochondrial oxygen uptake as functions of myoglobin or leghemoglobin
concentration. Closed symbols, myoglobin; open symbols, leghemoglobin
c. Different symbols represent different experiments. With the
exception of the two lowest points, oxygen uptake rates are those reported
spectrophotometrically by the near-steady-state rates of deoxygenation of
myoglobin or leghemoglobin, as in Fig.
3. Each point is the average of measurements on seven (myoglobin)
or two (leghemoglobin) preparations of isolated mitochondria. The two lowest
points were determined using trace concentrations of myoglobin or
leghemoglobin as a reporter of oxygen pressure. Oxygen uptakes are normalized
to a plateau value determined by the polarographically determined specific
activity. The ratios of the rates of oxygen uptake at the plateau of this
figure to those determined polarographically were 0.91, 0.77 and 1.30 in
experiments using myoglobin and 0.76, 0.96 and 0.92 in experiments using
leghemoglobin.
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