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Figure 2


Fig. 2. Oxygen pressure in a suspension of mitochondria reported polarographically as a function of time (1 torr=0.133 kPa). ADP (500 nmol) is added at the arrows. A, myoglobin absent; B, 500 µmol l–1 oxymyoglobin. Average oxygen uptake in the presence of adequate ADP is 193 and 157 mol O2 (mol cytochrome aa3)–1 min–1 in the absence and presence of myoglobin, respectively. P/O ratios are 2.21 and 2.20. The concentration of myoglobin used here exceeds the volume-average concentration in pigeon heart, 209 µmol l–1 (Schuder et al., 1979). These results show that myoglobin in the solution does not affect the respiratory parameters of isolated mitochondria.





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