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Figure 6


Fig. 6. Quantitative PCR analysis of mRNA encoding C and D forms of Na+/K+-ATPase {alpha}-subunit in individual gills of Pachygrapsus marmoratus transferred from 36{per thousand} seawater to 10{per thousand} (A,B) or to 45{per thousand} (C,D) seawater. Values are mean ± s.d. (N=3 measurements at each time point using gills pooled from three or four animals). Relative expression levels are indicated in relation to a standard reference (one sample of gill 6 at 48 h after transfer to 10{per thousand}). Significant differences from zero time values (animals in 36{per thousand} seawater) are indicated: *P<0.001; {dagger}P<0.01; {ddagger}P<0.05.





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