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Figure 5


Fig. 5. Quantitative PCR analysis of arginine kinase mRNA abundance in individual gills of Pachygrapsus marmoratus transferred from 36{per thousand} to 10{per thousand} (A) or to 45{per thousand} (B) seawater for time periods indicated above the figure. Mean ± s.d. (N=3 or 4 measurements at each time point using gills pooled from three or four animals). Relative expression levels are indicated in relation to a standard reference (one sample of gill 6 at 48 h after transfer to 10{per thousand}). Significant differences from zero time values (36{per thousand} seawater) are indicated: *P<0.001; {dagger}P<0.01; {ddagger}P<0.05.





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