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Figure 4


Fig. 4. Quantitative PCR analysis of Na+/K+-ATPase {alpha}-subunit mRNA abundance in individual gill preparations from Pachygrapsus marmoratus following transfer for various time periods (2–48 h) from 36{per thousand} seawater to 10{per thousand} (A,B) or to 45{per thousand} (C,D). Values are means ± s.d. (N=3 or 4 measurements at each time point using gills pooled from three or four animals) of data obtained with primers NAKPMF1 and NAKPMR1 designed to detect both isoforms simultaneously (Table 1). Two independent experiments from 2002 (A,C) and 2003 (B,D) are presented. Relative expression levels are indicated in relation to a standard reference (one sample of gill 6 at 48 h after transfer to 10{per thousand}). Significant differences from zero time values (crabs in 36{per thousand} seawater) are indicated: *P<0.001; {dagger}P<0.01.





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