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Fig. 1. Multiple alignment and phenogram of initial 707-nucleotide amplification
product of Na+/K+-ATPase
-subunit from
Pachygrapsus marmoratus (accession no. AF375957) with corresponding
partial cDNA sequences from four other arthropods: blue crab Callinectes
sapidus (acc. no. AF327439), American lobster Homarus americanus
(acc. no. AY140650), mosquito Anopheles gambiae (acc. no. XM_563283),
and honey bee Apis mellifera (acc. no. XM_392363). The alignment (A)
and phenogram (B) were both generated using the Hein algorithm
(Hein, 1990) in the MegAlign
component of DNASTAR software and configured with GeneDoc
(Nicholas and Nicholas, 1997).
The blue background indicates 100% agreement between all five sequences, green
80%, and yellow 60%. Bootstrap values at each node of the phenogram=100.