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Fig. 1. Set-up used to control the body temperature of the cicada during
intracellular recordings of auditory interneurons and recordings of the
auditory nerve activity. The temperature of the animal holder was modified
with a Peltier element and controlled via two thermocouples. The
sensor in the holder kept its temperature within values compatible with the
living tissues (1040°C) while the second thermocouple measured and
was used to control the temperature of the cicada body. The flowing water is
needed to add to or remove heat from the Peltier element.