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Figure 7


Fig. 7. Vacuolar H+-ATPase specific activity levels in different yolk granule (YG) fractions during days 0 and 3 of development. LYGs and SYGs were submitted to membrane fraction separation and assayed for H+-ATPase activity. (A) Vacuolar H+-ATPase activity levels during days 0 and 3 of embryogenesis. White bars represent day 0 activities and gray bars represent day 3 activities. Values are means ± s.e.m. of 3 different experiments. To investigate the location of the H+-ATPase, YGs were incubated with AO in the presence of ATP during day 0 (B,C) and day 3 (D,E) of embryogenesis. Arrows indicate acidic SYGs and arrowheads indicate acidic LYGs. (B,D) DIC images; (C,E) corresponding fluorescence images. Scale bars, 25 µm.





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