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Figure 3


Fig. 3. Induction of large yolk granule (LYG) formation by in vitro calcium treatment. (A) Formation of LYGs (>40 µm) as a function of calcium concentration. Incubation of day 0 SYGs with increasing concentrations of calcium lead to the formation LYGs (a 13-fold increase of LYGs can be observed in 23 mmol l-1 Ca2+). Values are means ± s.e.m. of 4 different experiments. *Statistically significant difference (P<0.05, one-way ANOVA). (B,C) DIC microscopy showing the general views of YGs incubated with 10 mmol l-1 EGTA (B) and ~23 mmol l-1 Ca2+ (C). Note the presence of a higher number of LYGs (arrow) after incubation with calcium. Scale bars, 200 µm.





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