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Fig. 10. Vitellin (VT) proteolysis after calcium incubation. (A) SDS-PAGE showing VT
proteolysis after calcium incubation. Yolk granules (YGs) were extracted from
day 0 and day 3 eggs and incubated in Ringer saline containing 1 mmol
l-1 ATP and 1 mmol l-1 PPi. For experimental groups
23 mmol l-1 Ca2+ was added. (Lane 1) Day-0 YGs
after incubation with EGTA; (lane 2) day-0 YGs after incubation with calcium;
(lane 3) day-3 YGs after incubation with EGTA; (lane 4) day-3 YGs after
incubation with calcium. (B) Cathepsin D activities tested in all samples used
in A. AU, arbitrary units.