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Fig. 8. Activation of p44ERK by serum (FBS) is dependent on Raf-1, but activation
of p44ERK during recovery is independent of Raf-1. (A) Western blot analysis
(top) and quantification (bottom) of phospho-p44ERK after serum stimulation of
serum-starved cells, in the absence and presence of 10 µmol
l1 Raf-1 inhibitor, RKI. (B) Western blot analysis (top) and
quantification (bottom) of the effect of 10 µmol l1 Raf-1
inhibitor on p44ERK phosphorylation during chemical anoxia and recovery (A/R)
(N=3, *P<0.05).