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Fig. 7. Reactivation of p44ERK under recovery requires PP1/PP2A activity. RTHDF
cells were treated with 100 nmol l1 calyculin A (Cal. A; an
inhibitor of PP1/2) for 2 min prior to treatment. Cell extracts were analysed
by western blotting (top) and the immunoreactive bands were quantified
(bottom). (A) Effect of calyculin A on normoxic p44ERK activity. (B) Effect of
calyculin A on p44ERK phosphorylation after chemical anoxia/recovery (A/R)
(N=3, *P<0.05).