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Fig. 3. Time-dependent inhibition of p44ERK activity during chemical anoxia and
activation during reoxygenation in RTHD fibroblasts. RTHDF cells were
challenged with chemical anoxia and recovery for the indicated periods of
time. Chemical anoxia was induced by incubation in L-15ex, containing 10 mmol
l–1 sodium azide for 30 min. Recovery was achieved by
incubating the cells in azide-free L-15ex. (A) Top: western blot analysis of
phospho-p44ERK during anoxia (N=4) and total p44ERK (N=1).
Bottom: quantification of band intensity showed significant inhibition of
p44ERK activity after 30 min, relative to the normoxic control. (B) Top:
western blot analysis of phospho-p44ERK during recovery (N=3) and
total p44ERK (N=1). Bottom: quantification of band intensity showed
that recovery resulted in significant activation of p44ERK after 10 min,
relative to anoxic cells. *P<0.005.
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