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Fig. 3. Focal brain application of 5-HT indirectly inhibits the swim-gating neuron
204 (recorded in M11). Cell 204 becomes hyperpolarized and its tonic firing
rate is suppressed in response to the brain application of 5-HT (5 µmol
l1) (middle), but not by octopamine (50 µmol
l1) (OA; bottom). During perfusion of amine-free saline
(top), cell 204 fired action potentials at a constant rate of about
10 Hz. A schematic of the experimental preparation, cell 204 recording site,
and placement of amine is shown to the left.