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Fig. 7. 5-HT-induced pH changes in the lumen of the salivary gland as measured with
double-barrelled pH-sensitive microelectrodes. (A) Effects of three different
5-HT concentrations (1, 3 and 10 nmol l–1) on transepithelial
potential (upper trace) and luminal pH (lower trace). The slightly positive
transepithelial potential in the unstimulated gland became at least
transiently negative upon 5-HT stimulation and displayed a large positive
overshoot after 5-HT withdrawal as described previously
(Berridge and Prince, 1971). In
our hands the time courses of the transepithelial potential changes recorded
by the reference barrel of the pH-sensitive microelectrodes differed slightly
from those recorded by the classical paraffin oil-gap method (e.g.
Berridge and Prince, 1971) for
unknown reasons. However, similar differences were noted earlier in luminal
recordings with double-barrelled K+-sensitive microelectrodes by
the same group (see e.g. fig. 5 in Gupta
et al., 1978). (B) Dose–response relationship of the
5-HT-induced pH changes obtained from recordings similar to those shown in
A.
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