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Figure 5


Fig. 5. Dose–response curve for 5-HT-induced changes in luminal surface pH. (A–C) Each salivary gland with HAF at its apical membrane domain was exposed first to 30 nmol l–1 5-HT (reference stimulus), then to a test stimulus of variable 5-HT concentration. An area of about 20 µmx20 µm, representing the size of one cell, was selected in the field of view to generate the graph. Note that the luminal compartment acidified reversibly in response to a 5-HT stimulus. (D) The decrease in F470/F410 ratio evoked by the test stimulus was normalized to the F470/F410 change induced by the reference stimulus. Values represent means ± s.d. of 5 experiments for each concentration.





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