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Figure 4


Fig. 4. Spatiotemporal analysis of pH changes at the luminal surface evoked by 30 nmol l–1 5-HT. (A) Series of pseudocolour images of the F470/F410 ratio in a salivary gland with HAF in the apical membrane. The squares in the first image indicate the areas selected to generate the graph. +5-HT, beginning of the 5-HT stimulus; –5-HT, washout of 5-HT. Note that the F470/F410 ratio laterally along the salivary gland tube (asterisks) is not linked to pH because it results from cellular autofluorescence rather than HAF fluorescence. Only F470/F410 in the mid-region of the salivary gland results from HAF and thus relates to luminal surface pH. Image intervals, 50 s; scale bar, 50 µm. (B) Time course of 5-HT-induced changes in luminal surface pH in four different regions along a salivary gland (squares in A).





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