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Fig. 3. pH-dependent changes in HAF fluorescence. A salivary gland was incubated
for 5 min with 30 µmol l–1 HAF in PS in order to label the
basolateral surface of the epithelial cells and was then superfused with PS of
different pH as indicated. (A) HAF fluorescence upon excitation at 470 nm and
410 nm. (B) Ratio of the fluorescence signals obtained at the excitation
wavelengths of 470 nm and 410 nm (F470/F410).
Acidification is accompanied by a decrease in the ratio and alkalization by an
increase in F470/F410.
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