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Fig. 2. HAF in the apical membrane domain. (A) Timed series of confocal images
through a live salivary gland after pressure-injection of 30 µmol
l–1 HAF in PS into its lumen. The first image was recorded
3 min after dye injection. Subsequent images were taken at an interval of
3.2 min. Note that fluorescence intensity gradually rises within the first
four images, indicating the insertion of more dye molecules into the membrane.
(B,C) Confocal images of a live salivary gland 20 min after injection of
HAF into its lumen (asterisks). The optical section plane is indicated (upper
right of each image). HAF staining is present on the luminal surface of the
secretory cells and extends into the canaliculi, which are deep infoldings of
the apical membrane (arrows). Double arrows indicate tracheoles that reside on
the outer surface of the gland and that exhibit autofluorescence. (D,E)
Confocal images through a chemically fixed, Oregon
Green–Phalloidin-stained salivary gland (shown for comparative
purposes). Phalloidin-labelled actin filaments line the canaliculi (long
arrows). Asterisks indicate the lumen of the gland, and broad arrows indicate
F-actin at the basal surface. Bars, 50 µm (A); 20 µm (B–E).
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