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Fig. 8. Collagen fibril aggregation assay in (a,b) Ca2+-containing
medium and (c,d) Ca2+-free medium. Addition of the medium
containing H-tensilin to the suspension caused the aggregation of
collagen fibrils into a clot (b,d) whereas addition of the medium alone did
not cause the aggregation (a,c). These dark-field light micrographs were taken
at low magnification. Concentrations of collagen fibrils were the same from a
to d. Scale bar, 500 µm.