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Figure 6


Fig. 6. Effects of 100 µmol l–1 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) (A), control treatment with neither DIDS nor solubilised apical plasma membrane protein fraction (sAPM) (B) and 50 µg ml–1 sAPM (C) on the intracellular pH of boar spermatozoa incubated for 1 h in the presence (filled circles) and absence (open squares) of bicarbonate/CO2. Whereas bicarbonate/CO2 induced increased pHi in all treatments, DIDS significantly inhibited (F1,252=93.3; P<0.001) and sAPM significantly enhanced (F1,356=52.45; P<0.001) the pH increase with respect to the control. (Data are means ± s.e.m.; N=6





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