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Figure 9


Fig. 9. Extracellular spike waveforms are responsive to predator odour. (A,B) Spikes during perfusion of control seawater (white) and seawater with predator odour (black) are overlaid. Control waveforms are drawn after predator odour waveforms and waveform transparency is scaled to the total number of waveforms displayed. Consequently, any distinct dark areas indicate waveforms with higher relative frequency during perfusion of predator odour. The number of waveforms displayed is given for each treatment type (controls, white; predator odour, black). (Ai–vii) All large amplitude spikes in the analysis window (the voltage level defining `large' is consistent for each rhinophore). (Bi–vii) Only waveforms that matched a template using Spike2 software. For each rhinophore tested (i–vii), amongst the various large magnitude waveforms recorded (A), a single group of dark waveforms with distinct shape can be seen, and can be sorted using templates (B). This waveform occurred either not at all, or at much lower frequency during perfusion of control seawater. In rhinophore vii, spontaneous unresponsive activity was too great to visualize the responsive waveform, and therefore waveforms matching the six most frequent templates are not drawn in A to avoid obscuring rarer waveforms. Scale bars (20 µV) apply to A and B for each rhinophore.





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