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Fig. 9. Extracellular spike waveforms are responsive to predator odour. (A,B)
Spikes during perfusion of control seawater (white) and seawater with predator
odour (black) are overlaid. Control waveforms are drawn after predator odour
waveforms and waveform transparency is scaled to the total number of waveforms
displayed. Consequently, any distinct dark areas indicate waveforms with
higher relative frequency during perfusion of predator odour. The number of
waveforms displayed is given for each treatment type (controls, white;
predator odour, black). (Aivii) All large amplitude spikes in the
analysis window (the voltage level defining `large' is consistent for each
rhinophore). (Bivii) Only waveforms that matched a template using
Spike2 software. For each rhinophore tested (ivii), amongst the various
large magnitude waveforms recorded (A), a single group of dark waveforms with
distinct shape can be seen, and can be sorted using templates (B). This
waveform occurred either not at all, or at much lower frequency during
perfusion of control seawater. In rhinophore vii, spontaneous unresponsive
activity was too great to visualize the responsive waveform, and therefore
waveforms matching the six most frequent templates are not drawn in A to avoid
obscuring rarer waveforms. Scale bars (20 µV) apply to A and B for each
rhinophore.