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Fig. 8. Measurement of HSP70 protein levels by immunoblot analysis under
extracellular alkaline (pH 8.5) conditions. (A) Protein (50 µg) from hearts
perfused with a Tris–Tyrode's solution of pH 8.5 for the indicated times
was immunoassayed with an anti-HSP70 antibody. Co, control. (B) Densitometric
analysis of HSP70 bands by laser scanning. Results are means ± s.e.m.
for three independent experiments. *P<0.05 vs control
value. (C) Effect of the p38-MAPK specific inhibitor SB203580 (1 µmol
l–1) on the protein levels of HSP70 in the presence of
extracellular alkalosis.
