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Fig. 2. Effect of the specific inhibitor SB203580 on the p38-MAPK phosphorylation
induced by extracellular alkalosis (pH 8.5). (A) Protein (50 µg) from
hearts perfused without (Co) or with an alkaline (pH 8.5) Tris–Tyrode's
solution for 2 min in the absence (–) or presence (+) of 1 µmol
l–1 SB203580 was assessed by immunoblot using phosphospecific
anti-p38-MAPK (top) or anti-actin antibody (bottom). (B) Densitometric
analysis of phospho-p38-MAPK bands by laser scanning. Results are means
± s.e.m. for three independent experiments. *P<0.05
vs control value.
