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Figure 7


Fig. 7. Spontaneous beating of basal region dissected flagella. A head-attached, reactivated spermatozoon was treated with PRODAN-UV at the basal region of flagellum. Then, the PRODAN-UV treated basal region (between the arrows) was dissected out by a microglass needle. The dissected flagellum continued to show sinusoidal wave movement without a head and base of flagellum. This experiment was repeated more than 20 times. ATP concentration was 15 µmol l–1. Bar, 10 µm. (A) Micrograph 1, the inhibited reactivated flagellum before its head was dissected out. Micrograph 2, the process of dissection. Micrograph 3–7, phase-contrast micrographs of the reactivated flagellum whose basal region (between two arrows) was PRODAN-UV treated and dissected out. Each micrograph represents 1/30 s. (B) Superimposed shear curves of the panels in A.





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