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Fig. 3. Dark-field micrographs (AC) and shear curves (DF) of
reactivated sea urchin sperm flagella. ATP concentration was 15 µmol
l1. (A) Dark-field micrographs of symmetrical bending waves
of the control sperm flagellum before UV irradiation. Beat frequency was 4.8
Hz. Bar, 10 µm. (B) Dark-field micrographs of `proximal region inhibition
step 1'. Approximately 5 µm of the proximal region was inhibited (between
the two arrowheads). Beat frequency was 5.0 Hz. Bar, 10 µm. (C) Dark-field
micrographs of `proximal region inhibition step 2'. Approximately 12 µm of
the proximal region was inhibited (between the two arrowheads). Beat frequency
was 8.0 Hz. Bar, 10 µm. (DF) Results obtained from the series of
seven frames in AC are superimposed in DF, respectively. Every
shear curve represents every 1/30 s. (D) Shear curves corresponding A. (E)
Shear curves corresponding B. (F) Shear curves corresponding C.