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Fig. 2. Experimental protocol for measuring
max and power output of the
in situ Atlantic salmon heart. The broken line represents the
end-diastolic pressure developed by the ventricle, determined by adjusting the
height of the output pressure head. Pout was normally set
to a physiologically realistic value of 5 kPa; however a subphysiological
level of Pout (23 kPa) was used at the start of the
protocol to let the heart recover from surgery. The first set of steps
indicate the maximum cardiac output test
(
max), where
Pin was raised sequentially from 0.15 kPa to 0.6 kPa,
whereas the second set of steps indicate the myocardial power test where
output pressure was raised from 3 kPa to 10 kPa, while Pin
remained at 0.60 kPa.