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Fig. 2. The relationships between breathing parameters as measured by analysis of
video recordings or from computerized data acquisition. (A) Correlation
between breathing frequencies (fR) determined by the two
methods based on analysis of six different fish exhibiting a wide range of
fR (r2=0.999,
y=0.986x+0.687). (B) Correlation between opercular
displacement (a measure of breathing amplitude) determined by the two methods
during normocapnia and hypercapnia (PwCO2=3.5 mmHg). Each
plot represents a change in amplitude of a single fish and each fish is
represented by a different symbol (N=6). (C) Changes in ventilation
amplitude during filming were analogous regardless of the method of
measurement; data plotted are taken from B (r2=0.968,
y=0.819x+0.119); N=6.